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admin
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Joined: 08 Dec 2003
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admin
Site Admin
Joined: 08 Dec 2003
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 Posted: Thu Jul 30, 2009 3:15 pm Post subject: |
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hua8986059
Joined: 10 Mar 2011
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| Posted: Wed May 04, 2011 2:23 pm Post subject: |
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| so Amino acids with acidic side chains would have isoelectric point of lower than 7 right? |
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mohsinalam5969
Joined: 18 Apr 2011
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| Posted: Sun Jul 03, 2011 11:41 am Post subject: |
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@hua8986059
The pI will be at a lower pH because the acidic side chain introduces an "extra" negative charge. So the neutral form exists under more acidic conditions when the extra -ve has been neutralised. For example, for aspartic acid the neutral form is dominant between pH 1.88 and 3.65, pI is halfway between these two values, i.e. pI = 1/2 (pKa1 + pKa3), so pI = 2.77.
Hope you understamd.Thanks |
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